MTA-12 and Custom Arrays

Assay Principle & Procedure

Product Specifications for:

MTA-12 / Custom Arrays

















Tricyclic Antidepressants

Multi-Test Array (MTA™)

Streamlined Drug Screening with Multi-Test Array (MTA™)

Screen for up to 12 drugs at once with Venture Lab’s innovative Multi-Test Array (MTA™). First of its kind in the field of the forensic diagnostics, MTA™ utilizes the array format on a microplate in combination with our proprietary enzyme-conjugate reagent to screen for 12 drugs with one easy procedure, all in under an hour.

Or, custom configure the Multi-Test Array to suit your testing needs by selecting any combination of 4 or more tests from our panel while keeping the easy one kit convenience. For example, a NHTSA panel
(DUI negative for alcohol) might include amphetamine, benzodiazepines, cocaine, methamphetamine, opiates and THC. A meconium sample from at risk neonates might include amphetamine, cocaine, opiates and THC.

How It Works

Each color-coded column of MTA wells contains antibodies to either amphetamine, barbiturates, benzodiazepines, buprenorphine, cannabinoids, cocaine, fentanyl, ketamine, MDMA, methadone, methamphetamine, opiates, oxycodone, phencyclidine, propoxyphene, tramadol or tricyclic antidepressants.

The unique combination enzyme-conjugate reagent contains all the chemistry necessary for up to 12 assays, making testing for 12 drugs simultaneously as easy as any single analyte kit.

Simply add calibrator or sample to each of the wells in a column to test for all 12 drugs. Next, add the combination enzyme conjugate reagent to all of the wells.

Example MTA-12 (any 12 assays may be combined on one plate)

Continue the assay as you would any simple microplate EIA: incubate, wash, add substrate, and stop. Testing for 12 drugs becomes as quick and easy as testing for one.

Flexible analysis with unsurpassed sensitivity

MTA™ assayys can be used to obtain quantitative results with a calibration range from 10 ng/mL to 80 ng/mL (1 ng/mL to 8 ng/mL for THC and PCP, 0.5 to 4ng/mL for buprenorphine and 50-400pg/mL for fentanyl). Optionally, an MTA™ kit can provide highly efficient qualitative screening of twelve drugs by selecting a cutoff calibrator within the assay range.

For quantitative results, calibrator absorbance values create a standard curve for plotting sample results. In effect, the blue color is inversely proportional to the amount of drug in each calibrator or sample, so more drug is present in the lighter wells.

In the example shown here, each row begins with 5 calibrators that form the standard curve for each drug. The calibrators span a range of 0 ng/mL to 80 ng/mL (1 to 8 ng/mL for THC and PCP). The wells after the calibrators contain samples.

* calibrators are 0ng/mL, 1ng/mL, 2ng/mL, 4ng/mL and 8ng/mL for Cannabinoids and Phencyclidine

The sample results for all of the drugs are interpreted as follows:

Sample #1 :

positive for amphetamine (20 ng/mL), negative for all others

Sample #2 :

positive for tricyclics (40 ng/mL) and oxycodone (40 ng/mL), negative for all others

Sample #3 :

positive for both benzodiazepines (40 ng/mL) and barbiturates (40 ng/mL), negative for all others

For qualitative results, an inspection of the microtiter plate example shown below allows a comparison with the cut-off calibrator to determine a positive or negative result for each drug. Cut-off calibrators can be set at any level within the assay range, and they can differ from drug to drug.

Sample #1 :

positive for opiates and amphetamine. negative for all others

Sample #2 :

positive for oxycodone, negative for all others

Sample #3 :

positive for cocaine, benzodiazepines, and barbiturates, negative for all others.

Specification data for MTA-12/Custom Arrays

Assay Protocol and Principle

Intended for forensic applications only.